Compatible with reusable counting boards, the bright field Countess 3 cell counter: Invitrogen Countess 3 fully automatic cell counter uses excellent machine learning image analysis algorithms to provide accurate cell counting and cell viability determination in less than 30 seconds. Countess 3 cell counting instrument has automatic lighting, focusing, capturing, counting, and saving functions; You only need to insert the cell counting plate containing the cell sample.
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1、 Product operating principle:
(1) Countess disposable cell counting board provides morebigThe convenience of limitations, Countess3 cell counter can achieve sustainability and cost reduction by reusing counting plates. Enjoy the speed and convenience brought by automation and counting performance.
(2) The Countess3FL fully automatic fluorescent cell counter is compatible with reusable counting boards and features flexible fluorescence and bright field dual-mode cell counting.
(3) The InvitrogenCountess3FL fully automatic cell counter has fluorescence function and can select two easily replaceable fluorescence cubes from 14 spectral range options. Like the Countess3 cell counting instrument, the Countess3FL fully automatic fluorescent cell counting instrument can also count cells and detect activity in the bright field. Using fluorescent reagents, it can also report fluorescent protein expression, apoptosis, transfection efficiency, and cell activity in just 30 seconds.
(4) The fluorescent cell counter also allows for the use of fluorescence based cell detection, which has stronger specificity for complex samples by using fluorescent cell dyes or fluorescent labeled cells.
2、 Common characteristics:
The common features of Countess3 and Countess3FL cell counters are accuracy, speed, simplicity, and automation;
Provide cell viability assays and single-cell data;
Compatible with reusable counting boards;
Optional software that supports compliance requirements for electronic records in 21CFR Part 11.
3、 Countess3 uses trypan blue for cell viability assay:
Use Countess3 fully automatic cell counter to count mouse PBMCs that are small in size, low in concentration, and usually difficult to count. In this bright field counting, 0.4% trypan blue staining was used to distinguish activity, with live cells remaining semi transparent and dead cells stained dark. The red outline (circle) represents dead cells, while the green outline represents live cells.
The Countess3 series automatic cell counting&analyzer introduces artificial intelligence to improve accuracy to a higher level.
4、 Product Description:
(1) ThermoFisher Scientific offers two high-performance cell counters to meet any laboratory needs.
(2)Invitrogen ™ Countess ™ 3 and Countess ™ The 3FL automatic cell counter utilizes artificial intelligence (AI) and applies deep learning neural network algorithms to achieve high-precision cell counting, even for challenging cell sample types. Our scientists have implanted machine learning algorithms into the system to help you accurately count cells, while also avoiding user to user differences caused by the use of manual hemocytometers.
(3) The Countess3 series automatic cell counter has functions such as automatic light intensity adjustment, automatic focusing, and automatic saving, which can help you save time.
5Countess 3 fully automatic cell counterCharacteristics and advantages:
• Accuracy - Applying high-precision machine learning algorithms to cell counting
Precision - automatic light intensity adjustment and automatic focusing can greatly reduce user operation and differences
• Time saving - Quick capture and auto save features help generate and save results quickly
• Convenient - Built in pre dilution and cell passage calculator, histogram and gating features, can be reported in PNG, JPG, TIFF, PDF, CSV, FSC and other formats. VI. Results:
Economy - Both Countess3 instrument models are compatible with Invitrogen ™ Countess ™ Reusable glass slides
• Flexibility - Countess3FL has a bright field and 2 replaceable fluorescence channels, with up to 20 different fluorescence combinations to meet various detection needs
7、 Comparison between CountessII and CountessIIFL automated cell analyzers:
Fast, simple, and economical bright field counting CountessII
Automated cell counter with bright field and dual channel optional fluorescence counting CountessIIFL
Automated cell counter
8Countess 3 fully automatic cell counterThe demonstration:
| Step 1 |
Step 2 |
Step 3 |
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| Mix equal portions of the sample with 0.4% trypan blue solution and add 10 µ L to a Countess disposable or reusable counting plate. |
Enable 'Rapid Capture' on the home screen. |
Insert the glass slide into the Countess 3 cell counting instrument until it clicks into place. |
Countess 3 performance data:
| Technical Specifications |
Processing time: |
| Sample concentration range: 1 x 10 ⁴ -1 x 10 𔑃 cells/mL |
| Particle diameter range: approximately 4-60 µ m |
| Cell diameter range: approximately 4-60 µ m |
| Required sample volume: 10 μ L |
| USB drive: 32 GB (compatible with FAT32, exFAT, or NTSF) |
| Optical components: 5-megapixel camera, 2.5x optical magnification |
| physical properties |
Instrument size: |
| 9 feet wide x 5 and a half feet deep x 9 feet high; 228.6 mm wide X 139.7 mm deep x 228.6 mm high |
| Weight: 8 lb; 17.6 kg |
| Counting area |
Area: 2.26 mm x 1.69 mm (3.82 mm ²)
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| Instrument accessories included |
USB drive used for storing and transferring data to a computer |
| Countess disposable slide holder |
| Countess reusable slide holder |
| Countess Cell Counting Plate Slips (50 pieces) |
| Power cord and 4 power plug adapters suitable for most countries |
| Quick Reference Card (QRC) |
| Sell accessories separately |
Countess reusable counting board |
| High power USB Wi Fi module (adapter) |
| Countess disposable cell counting plate |
| Taipan Blue dye |
| Countess SAE Software Solution |
| Countess 3 Standard Cell Counting Plate |
| Sampling results |
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| Counting mode |
bright field |
bright field and Featuring two selectable fluorescent channels that can be switched by users (with over 20 types of fluorescent cubes to choose from) |
| glass slide |
Disposable glass slide |
Reusable glass slides and Disposable glass slide |
| focus |
Auto focus, while providing manual focus option |
| user interface |
Intuitive touch screen, supporting mouse operation according to needs |
| processing time |
Less than 10 seconds |
Note: This product is only used for scientific research in universities, pharmaceuticals, chemical factories, laboratories, and other fields. It is not intended for the medical field. If you have any related needs, please consult our company.